HARNESSING THE POWER OF POLYHERBALS: BIOACTIVE COMPOUNDS AGAINST SELECTED MULTIDRUG-RESISTANT BACTERIAL PATHOGENS

Abstract

This research explores the use of polyherbal extracts from Eclipta prostrata, E. alba, and Tridax procumbens as a potent antibacterial solution, reflecting their significant role in contemporary medicine. Through Gas Chromatography-Mass Spectrum (GCMS) analysis, 45 distinct chemical compounds were identified in the ethanol extract, with the ethyl acetate extract revealing 35 different chemicals. These compounds' profiles suggested diverse chemical compositions, with some exhibiting promising antibacterial properties. The minimum inhibitory concentration (MIC) values against various bacterial strains indicated effective antibacterial activity, particularly against E. coli and P. mirabilis, with MICs as low as 6.25 µg/ml. The study emphasized three compounds, including 6-Acetyl-5-(4-fluorophenyl) purine, for their potential in drug development, attributed to their chemical properties such as the ability to form hydrogen bonds and favourable solubility and lipophilicity. This investigation underscores the therapeutic potential of polyherbal formulations, highlighting their value in addressing bacterial infections. The docking study demonstrates that 6-(3-fluorobenzylamino) purine exhibits a strong affinity towards ompC, murA, and ESBL proteins through various interactions, indicating its potential as an inhibitory agent against drug-resistant proteins in the Enterobacteriaceae family. The positive docking scores across different proteins and interaction types underscore the compound's versatility and potential effectiveness in combating antibiotic resistance. Further research could validate these in silico findings and explore the clinical applicability of such compounds in treating drug-resistant infections.