HARNESSING PROBIOTICS FOR ORTHODONTIC SUCCESS: AN IN VITRO EVALUATION OF THEIR IMPACT ON BIOFILM FORMATION AND ORAL MICROBIOME MODULATION TO ENHANCE TREATMENT OUTCOMES AND LONG-TERM ORAL HEALTH

Abstract

Objective: This study aims to evaluate the effectiveness of different probiotic strains in reducing biofilm biomass and altering biofilm properties on extracted teeth.

Methods: Extracted teeth were incubated with probiotic solutions including Lactobacillus casei, Bifidobacterium bifidum, and Lactobacillus acidophilus for a period of 7 days. The control group received no probiotic treatment. Biofilm biomass was quantified using optical density measurements, and biofilm thickness and density were analyzed through confocal laser scanning microscopy (CLSM). Statistical analyses were conducted to assess the significance of changes observed.

Results: All probiotic strains significantly reduced biofilm biomass compared to the control group. Bifidobacterium bifidum exhibited the most substantial reduction in biofilm biomass (54%), followed by Lactobacillus acidophilus (51%) and Lactobacillus casei (47%). CLSM analysis revealed that probiotic treatments also led to a significant reduction in biofilm thickness and density, with Bifidobacterium bifidum showing the greatest effect. The statistical analysis confirmed significant differences between the control and probiotic-treated groups.

Conclusion: Probiotic strains Lactobacillus casei, Bifidobacterium bifidum, and Lactobacillus acidophilus were effective in reducing biofilm biomass and altering biofilm properties on extracted teeth. Bifidobacterium bifidum was particularly effective, suggesting its potential as a valuable adjunct in orthodontic care to manage biofilm-related issues. Further research is needed to explore the clinical implications of these findings and the optimal probiotic strains for oral health applications.